Yes! Those that lie will get defensive. They will do everything in their power to deflect your attention away from themselves and will get angry that you are questioning their innocence. People who are telling the truth tend to go the opposite way and go on the offense. This will become obvious to you when you are trying to have a conversation with a person and they try to change the subject or move the conversation in a different direction.
Since studying body language I have noticed that this is done often by liars and is a major ref flag! I have another table that I will share shortly